Transfection Reagents
Takara Bio RETRONECTIN DISH 10 PLATES
Dish; Precoated with RetroNectin Human Recombinant Fibronectin Fragment; Source: E. coli expressing human fibronectin fragment CH-296; For retrovirus-mediated gene transduction; 10 dishes
Andwin Scientific JETPRIME DNA/SIRNA RGNT 5ML
The jetPRIME DNA and siRNA Transfection Reagent is made to guarantee high DNA transfection efficiency and excellent gene silencing. This product is very cost-effective as it uses less reagent and DNA and provides more gentle transfections in comparison to other competitors. It can be used with no difficulty and with serum and antibiotics. This can produce 15 reactions in 6-well plates and has a buffer volume of 5 mL. It is sold individually.
HORIZON DISCOVERY BIOSCIENCES LIMITED SIGLO GREEN INDICATOR, 20NMOL
siGLO Green transfection indicator,20nmol
Lonza Walkersville Cell Line Nucleofector Kit T
The Cell Line Nucleofector Kit T is one of our five Nucleofector Kits suited for transfecting cell lines in the Nucleofector 2b Device. This kit has been determined to be optimal for transfecting following cell types:A431A549Caco-2CHO-K1D1 ORL UVAHT-1080NALM-6 (DSMZ)NRKP815RBL-2H3T84TF-1U-87 MGThe respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies in difficult-to-transfect cell linesApplications:Transfection of cell numbers in the range of 2 x 10⁵ to 2 x 10⁷ cells Low-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution T0.5 mL Supplement 130 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
Lonza Walkersville Human Monocyte Nucleofector Kit
The Nucleofector 2b System (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This Nucleofector Kit is the optimal kit for efficient transfection of primary human monocytes.The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.Benefits:High transfection efficiencies for primary cellsCells maintain functionality post transfectionApplications:Transfection of cell numbers in the range of 2 × 10⁵ to 2 × 10⁷ cellsLow-throughput transfection in single cuvette formatContent:2.25 mL Nucleofector Solution for Human Monocytes0.5 mL Supplement 130 µg pmaxGFP Vector (0.5 μg/μl in 10 mM Tris pH 8.0)25 Aluminum cuvette (100 µL)25 Single use pipettes
New England Biolabs, Inc. Universal miRNA Cloning Linker – 5 µg
Linker/RNA Ligation Protocol: The Universal miRNA cloning linker has a 3' blocking group (amine) to prevent self ligation, circularization and ligation to RNA at the 5' end. It also contains the BanI restriction site compatible with the miRNA cloning protocol used by Bartel. For ligation, resuspend the lyophilized oligonucleotide in RNAse-free water to 100 M stock concentration. (A resuspension volume of 8.6 µl will give a 0.58ug/l or 100 µM stock solution). Ligations are carried out with T4 RNA Ligase I in the absence of ATP. Approximately 1 ug is typically used per reaction.
New England Biolabs, Inc. NEB Turbo Competent E. coli (High Efficiency) – 20 x 0.05 ml/tube
Chemically competent E. coli competent cells suitable for high efficiency transformation and rapid colony growth.
Agilent Technologies XL10-GOLD ULTRACOMP CELLS
XL10-Gold ultracompetent cells are created for transformation of large DNA molecules with high efficiency. Ideal for constructing plasmid DNA libraries, as they decrease size bias and produce larger, more complex plasmid libraries.
Genesee Scientific Corporation POLYPLUS-TRANSF FECTOVIR-AAV
Polyplus-Transfection 101000004 FectoVIR-AAV, Recombinant AAV Virus Production in Suspension Cells, 100ml/Unit
Genesee Scientific Corporation PEIPRO 1.5 ML
PEIpro is an optimized PEI-based transfection reagent for scalable virus production
Agilent Technologies BL21(DE3) COMPETENT CELLS
BL21(DE3) Competent Cells shows high-level protein expression, encodes T7 RNA polymerase under the control of the lacUV5 promoter. Used for non-toxic proteins.
Lonza Walkersville P3 Primary Cell 96-well Nucleofector Kit (960 RCT)
The P3 Primary Cell 96-well Nucleofector Kit is one of our five kits suited for medium-throughput transfection of primary cells when working with the 4D-Nucleofector 96-well Unit.Benefits:High transfection efficiencies for primary cellsCompatible with liquid handling systems for automated high-throughput electroporationConditions are transferable between 4D-Nucleofector System, 4D-Nucleofector 96-well Unit and 384-well Nucleofector SystemApplications:Transfection of low cell numbers in the range of 5 x 10⁴ to 1 x 10⁶ cellsHigh-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR librariesContent:22.5 mL P3 Primary Cell Nucleofector Solution5 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)10 x 96-well Nucleocuvette Plate (20 μl)